Department Overview

We determine the structures of biological macromolecules using crystallography and NMR.

We study the biophysical and biochemical properties of individual macromolecules and their complexes.

We develop new biotechnological and medical applications.

We train the next generation of scientists.

Structural Biology

The lab is fully equipped for high-throughput crystallization of proteins and their complexes using the Formulatrix Formulator, Crystal Phoenix and Tecan Freedom EVO robotics systems.

An in-house Rigaku MicroMax-007 HF X-ray generator, with an Oxford CryoStream system and a Saturn 944+ detector, is available for screening and optimisation of crystals. The generator is also equiipped with a Rigaku BioSAXS 2000 setup for scattering measurements on proteins and protein complexes in solution.

For more demanding data collection projects, we have access to a number of world-class synchrotron sources, including ESRF, Soleil, DESY/PETRA and Diamond.

The Jean Jeener NMR Centre is equipped with two state-of-the-art Varian spectrometers with proton resonance frequencies of 600 and 800MHz respectively. The former has a number of different probes for applications in organic chemistry and biochemistry, while the latter is equipped with a four-channel cryoprobe with enhanced sensitivity and salt-tolerance.

These instruments support a wide range of NMR experiments on biological macromolecules, ranging from interaction studies to complete structure determination projects. The expertise center can provide support for all steps ranging from the production of isotopically labelled protein samples to data analysis.