Publications

Found 24 results
Filters: Keyword is Substrate Specificity and Author is Jan Steyaert  [Clear All Filters]
2018
Lee, S., J. Choi, J. Mohanty, L. P. Sousa, F. Tome, E. Pardon, J. Steyaert, M. A. Lemmon, I. Lax, and J. Schlessinger, "Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling.", Nature, vol. 553, issue 7689, pp. 501-505, 2018 01 25.
2016
Hariharan, P., M. Andersson, X. Jiang, E. Pardon, J. Steyaert, R. H Kaback, and L. Guan, "Thermodynamics of Nanobody Binding to Lactose Permease.", Biochemistry, vol. 55, issue 42, pp. 5917-5926, 2016 Oct 25.
2015
Sounier, R., C. Mas, J. Steyaert, T. Laeremans, A. Manglik, W. Huang, B. K. Kobilka, H. Déméné, and S. Granier, "Propagation of conformational changes during μ-opioid receptor activation.", Nature, vol. 524, issue 7565, pp. 375-8, 2015 Aug 20.
2014
Somme, J., B. Van Laer, M. Roovers, J. Steyaert, W. Versées, and L. Droogmans, "Characterization of two homologous 2'-O-methyltransferases showing different specificities for their tRNA substrates.", RNA, vol. 20, issue 8, pp. 1257-71, 2014 Aug.
Ehrnstorfer, I. A., E. R. Geertsma, E. Pardon, J. Steyaert, and R. Dutzler, "Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.", Nat Struct Mol Biol, vol. 21, issue 11, pp. 990-6, 2014 Nov.
2007
Spaepen, S., W. Versées, D. Gocke, M. Pohl, J. Steyaert, and J. Vanderleyden, "Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense.", J Bacteriol, vol. 189, issue 21, pp. 7626-33, 2007 Nov.
Versées, W., S. Spaepen, J. Vanderleyden, and J. Steyaert, "The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism.", FEBS J, vol. 274, issue 9, pp. 2363-75, 2007 May.
Versées, W., S. Spaepen, M. D. H. Wood, F. J. Leeper, J. Vanderleyden, and J. Steyaert, "Molecular mechanism of allosteric substrate activation in a thiamine diphosphate-dependent decarboxylase.", J Biol Chem, vol. 282, issue 48, pp. 35269-78, 2007 Nov 30.
2006
Vandemeulebroucke, A., W. Versées, J. Steyaert, and J. N. Barlow, "Multiple transients in the pre-steady-state of nucleoside hydrolase reveal complex substrate binding, product base release, and two apparent rates of chemistry.", Biochemistry, vol. 45, issue 30, pp. 9307-18, 2006 Aug 1.
2005
Huysmans, G., A. Ranquin, L. Wyns, J. Steyaert, and P. Van Gelder, "Encapsulation of therapeutic nucleoside hydrolase in functionalised nanocapsules.", J Control Release, vol. 102, issue 1, pp. 171-9, 2005 Jan 20.
Loverix, S., P. Geerlings, M. McNaughton, K. Augustyns, A. Vandemeulebroucke, J. Steyaert, and W. Versées, "Substrate-assisted leaving group activation in enzyme-catalyzed N-glycosidic bond cleavage.", J Biol Chem, vol. 280, issue 15, pp. 14799-802, 2005 Apr 15.
2003
Versées, W., and J. Steyaert, "Catalysis by nucleoside hydrolases.", Curr Opin Struct Biol, vol. 13, issue 6, pp. 731-8, 2003 Dec.
Vandemeulebroucke, A., W. Versées, S. De Vos, E. Van Holsbeke, and J. Steyaert, "Pre-steady-state analysis of the nucleoside hydrolase of Trypanosoma vivax. Evidence for half-of-the-sites reactivity and rate-limiting product release.", Biochemistry, vol. 42, issue 44, pp. 12902-8, 2003 Nov 11.
2002
Versées, W., K. Decanniere, E. Van Holsbeke, N. Devroede, and J. Steyaert, "Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.", J Biol Chem, vol. 277, issue 18, pp. 15938-46, 2002 May 3.
Mignon, P., J. Steyaert, R. Loris, P. Geerlings, and S. Loverix, "A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study.", J Biol Chem, vol. 277, issue 39, pp. 36770-4, 2002 Sep 27.
2001
Versées, W., K. Decanniere, R. Pellé, J. Depoorter, E. Brosens, D. W. Parkin, and J. Steyaert, "Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax.", J Mol Biol, vol. 307, issue 5, pp. 1363-79, 2001 Apr 13.
1998
Loverix, S., A. Winquist, R. Strömberg, and J. Steyaert, "An engineered ribonuclease preferring phosphorothioate RNA.", Nat Struct Biol, vol. 5, issue 5, pp. 365-8, 1998 May.
Zegers, I., R. Loris, G. Dehollander, F. A Haikal, F. Poortmans, J. Steyaert, and L. Wyns, "Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.", Nat Struct Biol, vol. 5, issue 4, pp. 280-3, 1998 Apr.
1997
Steyaert, J., "A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.", Eur J Biochem, vol. 247, issue 1, pp. 1-11, 1997 Jul 1.
1995
Steyaert, J., and Y. Engelborghs, "A two-binding-site kinetic model for the ribonuclease-T1-catalysed transesterification of dinucleoside phosphate substrates.", Eur J Biochem, vol. 233, issue 1, pp. 140-4, 1995 Oct 1.
1994
Steyaert, J., A. F. Haikal, and L. Wyns, "Investigation of the functional interplay between the primary site and the subsite of RNase T1: kinetic analysis of single and multiple mutants for modified substrates.", Proteins, vol. 18, issue 4, pp. 318-23, 1994 Apr.
1991
Steyaert, J., C. Opsomer, L. Wyns, and P. Stanssens, "Quantitative analysis of the contribution of Glu46 and Asn98 to the guanosine specificity of ribonuclease T1.", Biochemistry, vol. 30, issue 2, pp. 494-9, 1991 Jan 15.
Steyaert, J., A. F. Haikal, L. Wyns, and P. Stanssens, "Subsite interactions of ribonuclease T1: Asn36 and Asn98 accelerate GpN transesterification through interactions with the leaving nucleoside N.", Biochemistry, vol. 30, issue 35, pp. 8666-70, 1991 Sep 3.
Steyaert, J., L. Wyns, and P. Stanssens, "Subsite interactions of ribonuclease T1: viscosity effects indicate that the rate-limiting step of GpN transesterification depends on the nature of N.", Biochemistry, vol. 30, issue 35, pp. 8661-5, 1991 Sep 3.