Publications

Found 49 results
Filters: Keyword is Mutagenesis, Site-Directed  [Clear All Filters]
2016
Lafaye, C., I. Van Molle, V. Tamu Dufe, K. Wahni, A. Boudier, P. Leroy, J-F. Collet, and J. Messens, "Sulfur Denitrosylation by an Engineered Trx-like DsbG Enzyme Identifies Nucleophilic Cysteine Hydrogen Bonds as Key Functional Determinant.", J Biol Chem, vol. 291, issue 29, pp. 15020-8, 2016 07 15.
2014
Liebens, V., V. Defraine, A. Van der Leyden, V. N. De Groote, C. Fierro, S. Beullens, N. Verstraeten, C. Kint, A. Jans, E. Frangipani, et al., "A putative de-N-acetylase of the PIG-L superfamily affects fluoroquinolone tolerance in Pseudomonas aeruginosa.", Pathog Dis, vol. 71, issue 1, pp. 39-54, 2014 Jun.
2012
Salmon, D., G. Vanwalleghem, Y. Morias, J. Denoeud, C. Krumbholz, F. Lhommé, S. Bachmaier, M. Kador, J. Gossmann, F. Braga Steh Dias, et al., "Adenylate cyclases of Trypanosoma brucei inhibit the innate immune response of the host.", Science, vol. 337, issue 6093, pp. 463-6, 2012 Jul 27.
De Jonge, N., M. Simic, L. Buts, S. Haesaerts, K. Roelants, A. Garcia-Pino, Y. Sterckx, H. De Greve, J. Lah, and R. Loris, "Alternative interactions define gyrase specificity in the CcdB family.", Mol Microbiol, vol. 84, issue 5, pp. 965-78, 2012 Jun.
Tiels, P., E. Baranova, K. Piens, C. De Visscher, G. Pynaert, W. Nerinckx, J. Stout, F. Fudalej, P. Hulpiau, S. Tännler, et al., "A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes.", Nat Biotechnol, vol. 30, issue 12, pp. 1225-31, 2012 Dec.
Villadangos, A. F., H-L. Fu, J. A. Gil, J. Messens, B. P. Rosen, and L. M. Mateos, "Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter.", J Biol Chem, vol. 287, issue 1, pp. 723-35, 2012 Jan 02.
Martinez-Rodriguez, S., A. Garcia-Pino, F. Javier Las Heras-Vázquez, J. María Clemente-Jiménez, F. Rodríguez-Vico, J. M. García-Ruiz, R. Loris, and J. Antonio Gavira, "Mutational and structural analysis of L-N-carbamoylase reveals new insights into a peptidase M20/M25/M40 family member.", J Bacteriol, vol. 194, issue 21, pp. 5759-68, 2012 Nov.
2009
Guharoy, M., and P. Chakrabarti, "Empirical estimation of the energetic contribution of individual interface residues in structures of protein-protein complexes.", J Comput Aided Mol Des, vol. 23, issue 9, pp. 645-54, 2009 Sep.
Vincke, C., R. Loris, D. Saerens, S. Martinez-Rodriguez, S. Muyldermans, and K. Conrath, "General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.", J Biol Chem, vol. 284, issue 5, pp. 3273-84, 2009 Jan 30.
2007
Roos, G., A. Garcia-Pino, K. Van Belle, E. Brosens, K. Wahni, G. Vandenbussche, L. Wyns, R. Loris, and J. Messens, "The conserved active site proline determines the reducing power of Staphylococcus aureus thioredoxin.", J Mol Biol, vol. 368, issue 3, pp. 800-11, 2007 May 4.
2006
Roos, G., E. Brosens, K. Wahni, A. Desmyter, S. Spinelli, L. Wyns, J. Messens, and R. Loris, "Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin.", Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 62, issue Pt 12, pp. 1255-8, 2006 Dec 1.
Roos, G., L. Buts, K. Van Belle, E. Brosens, P. Geerlings, R. Loris, L. Wyns, and J. Messens, "Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family.", J Mol Biol, vol. 360, issue 4, pp. 826-38, 2006 Jul 21.
2005
Saerens, D., M. Pellis, R. Loris, E. Pardon, M. Dumoulin, A. Matagne, L. Wyns, S. Muyldermans, and K. Conrath, "Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies.", J Mol Biol, vol. 352, issue 3, pp. 597-607, 2005 Sep 23.
Dao-Thi, M-H., L. Van Melderen, E. De Genst, H. Afif, L. Buts, L. Wyns, and R. Loris, "Molecular basis of gyrase poisoning by the addiction toxin CcdB.", J Mol Biol, vol. 348, issue 5, pp. 1091-102, 2005 May 20.
Bozoky, Z., A. Alexa, P. Tompa, and P. Friedrich, "Multiple interactions of the 'transducer' govern its function in calpain activation by Ca2+.", Biochem J, vol. 388, issue Pt 3, pp. 741-4, 2005 Jun 15.
2004
Alexa, A., Z. Bozoky, A. Farkas, P. Tompa, and P. Friedrich, "Contribution of distinct structural elements to activation of calpain by Ca2+ ions.", J Biol Chem, vol. 279, issue 19, pp. 20118-26, 2004 May 7.
Messens, J., I. Van Molle, P. Vanhaesebrouck, M. Limbourg, K. Van Belle, K. Wahni, J. C. Martins, R. Loris, and L. Wyns, "How thioredoxin can reduce a buried disulphide bond.", J Mol Biol, vol. 339, issue 3, pp. 527-37, 2004 Jun 4.
Versées, W., S. Loverix, A. Vandemeulebroucke, P. Geerlings, and J. Steyaert, "Leaving group activation by aromatic stacking: an alternative to general acid catalysis.", J Mol Biol, vol. 338, issue 1, pp. 1-6, 2004 Apr 16.
Tompa, P., P. Buzder-Lantos, Á. Tantos, A. Farkas, A. Szilágyi, Z. Bánóczi, F. Hudecz, and P. Friedrich, "On the sequential determinants of calpain cleavage.", J Biol Chem, vol. 279, issue 20, pp. 20775-85, 2004 May 14.
Messens, J., I. Van Molle, P. Vanhaesebrouck, K. Van Belle, K. Wahni, J. C. Martins, L. Wyns, and R. Loris, "The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct.", Acta Crystallogr D Biol Crystallogr, vol. 60, issue Pt 6, pp. 1180-4, 2004 Jun.
2003
Azuaga, A. I., D. Canet, G. Smeenk, R. Berends, F. Titgemeijer, R. Duurkens, P. L. Mateo, R. M. Scheek, G. T. Robillard, C. M. Dobson, et al., "Characterization of single-tryptophan mutants of histidine-containing phosphocarrier protein: evidence for local rearrangements during folding from high concentrations of denaturant.", Biochemistry, vol. 42, issue 17, pp. 4883-95, 2003 May 6.
Derix, N. M., R. Wechselberger, M. A. van der Horst, K. J. Hellingwerf, R. Boelens, R. Kaptein, and N. A. J. van Nuland, "Lack of negative charge in the E46Q mutant of photoactive yellow protein prevents partial unfolding of the blue-shifted intermediate.", Biochemistry, vol. 42, issue 49, pp. 14501-6, 2003 Dec 16.
Canet, D., C. E. Lyon, R. M. Scheek, G. T. Robillard, C. M. Dobson, P. J. Hore, and N. A. J. van Nuland, "Rapid formation of non-native contacts during the folding of HPr revealed by real-time photo-CIDNP NMR and stopped-flow fluorescence experiments.", J Mol Biol, vol. 330, issue 2, pp. 397-407, 2003 Jul 4.
Lah, N., J. Lah, I. Zegers, L. Wyns, and J. Messens, "Specific potassium binding stabilizes pI258 arsenate reductase from Staphylococcus aureus.", J Biol Chem, vol. 278, issue 27, pp. 24673-9, 2003 Jul 4.
2002
Messens, J., J. C. Martins, K. Van Belle, E. Brosens, A. Desmyter, M. De Gieter, J-M. Wieruszeski, R. Willem, L. Wyns, and I. Zegers, "All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade.", Proc Natl Acad Sci U S A, vol. 99, issue 13, pp. 8506-11, 2002 Jun 25.

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