Publications

Found 14 results
Filters: Keyword is Mutagenesis, Site-Directed and Author is Wyns, Lode  [Clear All Filters]
2007
Roos, G., A. Garcia-Pino, K. Van Belle, E. Brosens, K. Wahni, G. Vandenbussche, L. Wyns, R. Loris, and J. Messens, "The conserved active site proline determines the reducing power of Staphylococcus aureus thioredoxin.", J Mol Biol, vol. 368, issue 3, pp. 800-11, 2007 May 4.
2006
Roos, G., E. Brosens, K. Wahni, A. Desmyter, S. Spinelli, L. Wyns, J. Messens, and R. Loris, "Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin.", Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 62, issue Pt 12, pp. 1255-8, 2006 Dec 1.
Roos, G., L. Buts, K. Van Belle, E. Brosens, P. Geerlings, R. Loris, L. Wyns, and J. Messens, "Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family.", J Mol Biol, vol. 360, issue 4, pp. 826-38, 2006 Jul 21.
2005
Saerens, D., M. Pellis, R. Loris, E. Pardon, M. Dumoulin, A. Matagne, L. Wyns, S. Muyldermans, and K. Conrath, "Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies.", J Mol Biol, vol. 352, issue 3, pp. 597-607, 2005 Sep 23.
Dao-Thi, M-H., L. Van Melderen, E. De Genst, H. Afif, L. Buts, L. Wyns, and R. Loris, "Molecular basis of gyrase poisoning by the addiction toxin CcdB.", J Mol Biol, vol. 348, issue 5, pp. 1091-102, 2005 May 20.
2004
Messens, J., I. Van Molle, P. Vanhaesebrouck, M. Limbourg, K. Van Belle, K. Wahni, J. C. Martins, R. Loris, and L. Wyns, "How thioredoxin can reduce a buried disulphide bond.", J Mol Biol, vol. 339, issue 3, pp. 527-37, 2004 Jun 4.
Messens, J., I. Van Molle, P. Vanhaesebrouck, K. Van Belle, K. Wahni, J. C. Martins, L. Wyns, and R. Loris, "The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct.", Acta Crystallogr D Biol Crystallogr, vol. 60, issue Pt 6, pp. 1180-4, 2004 Jun.
2003
Lah, N., J. Lah, I. Zegers, L. Wyns, and J. Messens, "Specific potassium binding stabilizes pI258 arsenate reductase from Staphylococcus aureus.", J Biol Chem, vol. 278, issue 27, pp. 24673-9, 2003 Jul 4.
2002
Messens, J., J. C. Martins, K. Van Belle, E. Brosens, A. Desmyter, M. De Gieter, J-M. Wieruszeski, R. Willem, L. Wyns, and I. Zegers, "All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade.", Proc Natl Acad Sci U S A, vol. 99, issue 13, pp. 8506-11, 2002 Jun 25.
1999
Hamelryck, T. W., R. Loris, J. Bouckaert, M-H. Dao-Thi, G. Strecker, A. Imberty, E. Fernandez, L. Wyns, and M. Etzler, "Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus.", J Mol Biol, vol. 286, issue 4, pp. 1161-77, 1999 Mar 5.
1998
Dao-Thi, M-H., L. Wyns, F. Poortmans, E. M. Bahassi, M. Couturier, and R. Loris, "Crystallization of ccdB.", Acta Crystallogr D Biol Crystallogr, vol. 54, issue Pt 5, pp. 975-81, 1998 Sep 1.
Loris, R., T. W. Hamelryck, J. Bouckaert, and L. Wyns, "Legume lectin structure.", Biochim Biophys Acta, vol. 1383, issue 1, pp. 9-36, 1998 Mar 3.
1996
Doumen, J., M. Gonciarz, I. Zegers, R. Loris, L. Wyns, and J. Steyaert, "A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.", Protein Sci, vol. 5, issue 8, pp. 1523-30, 1996 Aug.
1993
Steyaert, J., and L. Wyns, "Functional interactions among the His40, Glu58 and His92 catalysts of ribonuclease T1 as studied by double and triple mutants.", J Mol Biol, vol. 229, issue 3, pp. 770-81, 1993 Feb 5.