Publications

Found 10 results
Filters: Keyword is Mutagenesis, Site-Directed and Author is Jan Steyaert  [Clear All Filters]
2004
Versées, W., S. Loverix, A. Vandemeulebroucke, P. Geerlings, and J. Steyaert, "Leaving group activation by aromatic stacking: an alternative to general acid catalysis.", J Mol Biol, vol. 338, issue 1, pp. 1-6, 2004 Apr 16.
2002
Versées, W., K. Decanniere, E. Van Holsbeke, N. Devroede, and J. Steyaert, "Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.", J Biol Chem, vol. 277, issue 18, pp. 15938-46, 2002 May 3.
2001
De Vos, S., J. Backmann, M. Prévost, J. Steyaert, and R. Loris, "Hydrophobic core manipulations in ribonuclease T1.", Biochemistry, vol. 40, issue 34, pp. 10140-9, 2001 Aug 28.
2000
Loverix, S., A. Winqvist, R. Strömberg, and J. Steyaert, "Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond.", Chem Biol, vol. 7, issue 8, pp. 651-8, 2000 Aug.
1999
Langhorst, U., R. Loris, V. P. Denisov, J. Doumen, P. Roose, D. Maes, B. Halle, and J. Steyaert, "Dissection of the structural and functional role of a conserved hydration site in RNase T1.", Protein Sci, vol. 8, issue 4, pp. 722-30, 1999 Apr.
Huyghues-Despointes, B. M., U. Langhorst, J. Steyaert, C. N. Pace, and J. M. Scholtz, "Hydrogen-exchange stabilities of RNase T1 and variants with buried and solvent-exposed Ala --> Gly mutations in the helix.", Biochemistry, vol. 38, issue 50, pp. 16481-90, 1999 Dec 14.
1998
De Vos, S., J. Doumen, U. Langhorst, and J. Steyaert, "Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position.", J Mol Biol, vol. 275, issue 4, pp. 651-61, 1998 Jan 30.
Loverix, S., A. Winquist, R. Strömberg, and J. Steyaert, "An engineered ribonuclease preferring phosphorothioate RNA.", Nat Struct Biol, vol. 5, issue 5, pp. 365-8, 1998 May.
1996
Doumen, J., M. Gonciarz, I. Zegers, R. Loris, L. Wyns, and J. Steyaert, "A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.", Protein Sci, vol. 5, issue 8, pp. 1523-30, 1996 Aug.
1993
Steyaert, J., and L. Wyns, "Functional interactions among the His40, Glu58 and His92 catalysts of ribonuclease T1 as studied by double and triple mutants.", J Mol Biol, vol. 229, issue 3, pp. 770-81, 1993 Feb 5.