Publications

Found 131 results
Filters: Author is Jan Steyaert  [Clear All Filters]
2001
Versées, W., K. Decanniere, R. Pellé, J. Depoorter, E. Brosens, D. W. Parkin, and J. Steyaert, "Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax.", J Mol Biol, vol. 307, issue 5, pp. 1363-79, 2001 Apr 13.
2000
Langhorst, U., J. Backmann, R. Loris, and J. Steyaert, "Analysis of a water mediated protein-protein interactions within RNase T1.", Biochemistry, vol. 39, issue 22, pp. 6586-93, 2000 Jun 6.
Loverix, S., A. Winqvist, R. Strömberg, and J. Steyaert, "Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond.", Chem Biol, vol. 7, issue 8, pp. 651-8, 2000 Aug.
1999
Loris, R., U. Langhorst, S. De Vos, K. Decanniere, J. Bouckaert, D. Maes, T. R. Transue, and J. Steyaert, "Conserved water molecules in a large family of microbial ribonucleases.", Proteins, vol. 36, issue 1, pp. 117-34, 1999 Jul 1.
Langhorst, U., R. Loris, V. P. Denisov, J. Doumen, P. Roose, D. Maes, B. Halle, and J. Steyaert, "Dissection of the structural and functional role of a conserved hydration site in RNase T1.", Protein Sci, vol. 8, issue 4, pp. 722-30, 1999 Apr.
Huyghues-Despointes, B. M., U. Langhorst, J. Steyaert, C. N. Pace, and J. M. Scholtz, "Hydrogen-exchange stabilities of RNase T1 and variants with buried and solvent-exposed Ala --> Gly mutations in the helix.", Biochemistry, vol. 38, issue 50, pp. 16481-90, 1999 Dec 14.
1998
De Vos, S., J. Doumen, U. Langhorst, and J. Steyaert, "Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position.", J Mol Biol, vol. 275, issue 4, pp. 651-61, 1998 Jan 30.
Loverix, S., A. Winquist, R. Strömberg, and J. Steyaert, "An engineered ribonuclease preferring phosphorothioate RNA.", Nat Struct Biol, vol. 5, issue 5, pp. 365-8, 1998 May.
Dao-Thi, M-H., T. R. Transue, R. Pellé, N. B. Murphy, F. Poortmans, and J. Steyaert, "Expression, purification, crystallization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei.", Acta Crystallogr D Biol Crystallogr, vol. 54, issue Pt 5, pp. 1046-8, 1998 Sep 1.
Zegers, I., R. Loris, G. Dehollander, F. A Haikal, F. Poortmans, J. Steyaert, and L. Wyns, "Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.", Nat Struct Biol, vol. 5, issue 4, pp. 280-3, 1998 Apr.
Loverix, S., G. Laus, J. C. Martins, L. Wyns, and J. Steyaert, "Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis.", Eur J Biochem, vol. 257, issue 1, pp. 286-90, 1998 Oct 1.
1997
Loverix, S., J. Doumen, and J. Steyaert, "Additivity of protein-guanine interactions in ribonuclease T1.", J Biol Chem, vol. 272, issue 15, pp. 9635-9, 1997 Apr 11.
Steyaert, J., "A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.", Eur J Biochem, vol. 247, issue 1, pp. 1-11, 1997 Jul 1.
Steyaert, J., S. Umans, D. Willekens, E. Legius, E. Pijkels, C. de Die-Smulders, H. Van den Berghe, and J. P. Fryns, "A study of the cognitive and psychological profile in 16 children with congenital or juvenile myotonic dystrophy.", Clin Genet, vol. 52, issue 3, pp. 135-41, 1997 Sep.
1996
Doumen, J., M. Gonciarz, I. Zegers, R. Loris, L. Wyns, and J. Steyaert, "A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.", Protein Sci, vol. 5, issue 8, pp. 1523-30, 1996 Aug.
1995
Fish, W. R., Z. M. Nkhungulu, C. W. Muriuki, D. M. Ndegwa, J. D. Lonsdale-Eccles, and J. Steyaert, "Primary structure and partial characterization of a life-cycle-regulated cysteine protease from Trypanosoma (Nannomonas) congolense.", Gene, vol. 161, issue 1, pp. 125-8, 1995 Aug 8.
Steyaert, J., and Y. Engelborghs, "A two-binding-site kinetic model for the ribonuclease-T1-catalysed transesterification of dinucleoside phosphate substrates.", Eur J Biochem, vol. 233, issue 1, pp. 140-4, 1995 Oct 1.
1994
Pletinckx, J., J. Steyaert, I. Zegers, H. W. Choe, U. Heinemann, and L. Wyns, "Crystallographic study of Glu58Ala RNase T1 x 2'-guanosine monophosphate at 1.9-A resolution.", Biochemistry, vol. 33, issue 7, pp. 1654-62, 1994 Feb 22.
Steyaert, J., A. F. Haikal, and L. Wyns, "Investigation of the functional interplay between the primary site and the subsite of RNase T1: kinetic analysis of single and multiple mutants for modified substrates.", Proteins, vol. 18, issue 4, pp. 318-23, 1994 Apr.
1993
Loris, R., J. Steyaert, D. Maes, J. Lisgarten, R. Pickersgill, and L. Wyns, "Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin.", Biochemistry, vol. 32, issue 51, pp. 14229, 1993 Dec 28.
Loris, R., J. Steyaert, D. Maes, J. Lisgarten, R. Pickersgill, and L. Wyns, "Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin.", Biochemistry, vol. 32, issue 34, pp. 8772-81, 1993 Aug 31.
Bernard, P., K. E. Kézdy, L. Van Melderen, J. Steyaert, L. Wyns, M. L. Pato, P. N. Higgins, and M. Couturier, "The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase.", J Mol Biol, vol. 234, issue 3, pp. 534-41, 1993 Dec 5.
Steyaert, J., and L. Wyns, "Functional interactions among the His40, Glu58 and His92 catalysts of ribonuclease T1 as studied by double and triple mutants.", J Mol Biol, vol. 229, issue 3, pp. 770-81, 1993 Feb 5.
Steyaert, J., L. Van Melderen, P. Bernard, M. H. Thi, R. Loris, L. Wyns, and M. Couturier, "Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.", J Mol Biol, vol. 231, issue 2, pp. 513-5, 1993 May 20.
1992
Steyaert, J., A. F. Haikal, P. Stanssens, and L. Wyns, "Dissection of the ribonuclease T1 subsite. The transesterification kinetics of Asn36Ala and Asn98Ala ribonuclease T1 for minimal dinucleoside phosphates.", Eur J Biochem, vol. 203, issue 3, pp. 551-5, 1992 Feb 1.

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