Title | The Corynebacterium glutamicum mycothiol peroxidase is a reactive oxygen species-scavenging enzyme that shows promiscuity in thiol redox control. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Pedre, B., I. Van Molle, A. F. Villadangos, K. Wahni, D. Vertommen, L. Turell, H. Erdogan, L. M. Mateos, and J. Messens |
Journal | Mol Microbiol |
Volume | 96 |
Issue | 6 |
Pagination | 1176-91 |
Date Published | 2015 Jun |
ISSN | 1365-2958 |
Keywords | Bacterial Proteins, Corynebacterium glutamicum, Cysteine, Disulfides, Glycopeptides, Hydrogen Peroxide, Inositol, Kinetics, Molecular Sequence Data, Oxidation-Reduction, Oxidative Stress, Oxidoreductases, Peroxidases, Reactive Oxygen Species, Sequence Analysis, Protein, Sulfhydryl Compounds, Thioredoxins |
Abstract | Cysteine glutathione peroxidases (CysGPxs) control oxidative stress levels by reducing hydroperoxides at the expense of cysteine thiol (-SH) oxidation, and the recovery of their peroxidatic activity is generally accomplished by thioredoxin (Trx). Corynebacterium glutamicum mycothiol peroxidase (Mpx) is a member of the CysGPx family. We discovered that its recycling is controlled by both the Trx and the mycothiol (MSH) pathway. After H2 O2 reduction, a sulfenic acid (-SOH) is formed on the peroxidatic cysteine (Cys36), which then reacts with the resolving cysteine (Cys79), forming an intramolecular disulfide (S-S), which is reduced by Trx. Alternatively, the sulfenic acid reacts with MSH and forms a mixed disulfide. Mycoredoxin 1 (Mrx1) reduces the mixed disulfide, in which Mrx1 acts in combination with MSH and mycothiol disulfide reductase as a biological relevant monothiol reducing system. Remarkably, Trx can also take over the role of Mrx1 and reduce the Mpx-MSH mixed disulfide using a dithiol mechanism. Furthermore, Mpx is important for cellular survival under H2 O2 stress, and its gene expression is clearly induced upon H2 O2 challenge. These findings add a new dimension to the redox control and the functioning of CysGPxs in general. |
DOI | 10.1111/mmi.12998 |
Alternate Journal | Mol. Microbiol. |
PubMed ID | 25766783 |
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