Corynebacterium diphtheriae methionine sulfoxide reductase a exploits a unique mycothiol redox relay mechanism.

TitleCorynebacterium diphtheriae methionine sulfoxide reductase a exploits a unique mycothiol redox relay mechanism.
Publication TypeJournal Article
Year of Publication2015
AuthorsTossounian, M-A., Pedre B., Wahni K., Erdogan H., Vertommen D., Van Molle I., and Messens J.
JournalJ Biol Chem
Volume290
Issue18
Pagination11365-75
Date Published2015 May 01
ISSN1083-351X
KeywordsAmino Acid Sequence, Catalytic Domain, Corynebacterium diphtheriae, Cysteine, Disulfides, Glycopeptides, Inositol, Methionine, Methionine Sulfoxide Reductases, Models, Molecular, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases, Thioredoxin-Disulfide Reductase, Thioredoxins
Abstract

Methionine sulfoxide reductases are conserved enzymes that reduce oxidized methionines in proteins and play a pivotal role in cellular redox signaling. We have unraveled the redox relay mechanisms of methionine sulfoxide reductase A of the pathogen Corynebacterium diphtheriae (Cd-MsrA) and shown that this enzyme is coupled to two independent redox relay pathways. Steady-state kinetics combined with mass spectrometry of Cd-MsrA mutants give a view of the essential cysteine residues for catalysis. Cd-MsrA combines a nucleophilic cysteine sulfenylation reaction with an intramolecular disulfide bond cascade linked to the thioredoxin pathway. Within this cascade, the oxidative equivalents are transferred to the surface of the protein while releasing the reduced substrate. Alternatively, MsrA catalyzes methionine sulfoxide reduction linked to the mycothiol/mycoredoxin-1 pathway. After the nucleophilic cysteine sulfenylation reaction, MsrA forms a mixed disulfide with mycothiol, which is transferred via a thiol disulfide relay mechanism to a second cysteine for reduction by mycoredoxin-1. With x-ray crystallography, we visualize two essential intermediates of the thioredoxin relay mechanism and a cacodylate molecule mimicking the substrate interactions in the active site. The interplay of both redox pathways in redox signaling regulation forms the basis for further research into the oxidative stress response of this pathogen.

DOI10.1074/jbc.M114.632596
Alternate JournalJ. Biol. Chem.
PubMed ID25752606
PubMed Central IDPMC4416841
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