DYn-2 Based Identification of Arabidopsis Sulfenomes.

TitleDYn-2 Based Identification of Arabidopsis Sulfenomes.
Publication TypeJournal Article
Year of Publication2015
AuthorsAkter, S., Huang J., Bodra N., De Smet B., Wahni K., Rombaut D., Pauwels J., Gevaert K., Carroll K., Van Breusegem F., and Messens J.
JournalMol Cell Proteomics
Volume14
Issue5
Pagination1183-200
Date Published2015 May
ISSN1535-9484
KeywordsArabidopsis, Arabidopsis Proteins, Avidin, Biotin, Cell Compartmentation, Cell Culture Techniques, Cyclohexanones, Hydrogen Peroxide, Molecular Probes, Molecular Sequence Annotation, Organelles, Oxidation-Reduction, Oxidative Stress, Protein Processing, Post-Translational, Proteomics, Signal Transduction, Sulfenic Acids
Abstract

Identifying the sulfenylation state of stressed cells is emerging as a strategic approach for the detection of key reactive oxygen species signaling proteins. Here, we optimized an in vivo trapping method for cysteine sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells using a dimedone based DYn-2 probe. We demonstrated that DYn-2 specifically detects sulfenylation events in an H2O2 dose- and time-dependent way. With mass spectrometry, we identified 226 sulfenylated proteins after H2O2 treatment of Arabidopsis cells, residing in the cytoplasm (123); plastid (68); mitochondria (14); nucleus (10); endoplasmic reticulum, Golgi and plasma membrane (7) and peroxisomes (4). Of these, 123 sulfenylated proteins have never been reported before to undergo cysteine oxidative post-translational modifications in plants. All in all, with this DYn-2 approach, we have identified new sulfenylated proteins, and gave a first glance on the locations of the sulfenomes of Arabidopsis thaliana.

DOI10.1074/mcp.M114.046896
Alternate JournalMol. Cell Proteomics
PubMed ID25693797
PubMed Central IDPMC4424392
Grant ListR01 CA174864 / CA / NCI NIH HHS / United States
R01 GM102187 / GM / NIGMS NIH HHS / United States
CA174986 / CA / NCI NIH HHS / United States
GM102187 / GM / NIGMS NIH HHS / United States
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