|Title||Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Newman, J. A., Savitsky P., Allerston C. K., Bizard A. H., Özer Ö., Sarlós K., Liu Y., Pardon E., Steyaert J., Hickson I. D., and Gileadi O.|
|Journal||Nucleic Acids Res|
|Date Published||2015 May 26|
|Keywords||Adenosine Diphosphate, Crystallography, X-Ray, DNA, Models, Molecular, Mutation, Protein Conformation, Protein Structure, Tertiary, RecQ Helicases, Single-Domain Antibodies, Zinc|
Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.
|Alternate Journal||Nucleic Acids Res.|
|PubMed Central ID||PMC4446433|
|Grant List||092809/Z/10/Z / / Wellcome Trust / United Kingdom|
Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.