Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module.

TitleCrystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module.
Publication TypeJournal Article
Year of Publication2015
AuthorsHadži, S., A. Garcia-Pino, K. Gerdes, J. Lah, and R. Loris
JournalActa Crystallogr F Struct Biol Commun
Volume71
IssuePt 2
Pagination226-33
Date Published2015 Feb
ISSN2053-230X
KeywordsAntitoxins, Bacterial Proteins, Bacterial Toxins, Crystallization, Crystallography, X-Ray, DNA, Operator Regions, Genetic, Vibrio cholerae
Abstract

The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221, with unit-cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P43212 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P41212, with unit-cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.

DOI10.1107/S2053230X15000746
Alternate JournalActa Crystallogr F Struct Biol Commun
PubMed ID25664801
PubMed Central IDPMC4321481
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