Quaternary structure of UEA-II, the chitobiose specific lectin from gorse.

TitleQuaternary structure of UEA-II, the chitobiose specific lectin from gorse.
Publication TypeJournal Article
Year of Publication1998
AuthorsDao-Thi, M-H., P. Rizkallah, L. Wyns, F. Poortmans, and R. Loris
JournalActa Crystallogr D Biol Crystallogr
IssuePt 5
Date Published1998 Sep 1
KeywordsCarbohydrate Metabolism, Carbohydrate Sequence, Crystallization, Crystallography, X-Ray, Fabaceae, Lectins, Models, Molecular, Molecular Sequence Data, Plant Lectins, Plants, Medicinal, Protein Conformation, Substrate Specificity

The chitobiose specific Ulex europaeus lectin II crystallizes in space group P3221 with unit-cell dimensions a = b = 105.54, c = 176.26 A. The asymmetric unit contains a complete lectin tetramer. The crystals were shown to diffract to 4.5 A on a rotating-anode source and to 2.7 A at the Daresbury synchrotron source. Molecular replacement and subsequent rigid-body refinement using data to 4.5 A yielded a solution corresponding to a tetramer very similar to that of phytohemagglutinin-L and soybean agglutinin. The monomers in the Ulex lectin tetramer are rotated approximately 5 degrees compared with the phytohemagglutinin-L and soybean agglutinin structures.

Alternate JournalActa Crystallogr. D Biol. Crystallogr.
PubMed ID9757099