Title | Quaternary structure of UEA-II, the chitobiose specific lectin from gorse. |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | Dao-Thi, M-H., P. Rizkallah, L. Wyns, F. Poortmans, and R. Loris |
Journal | Acta Crystallogr D Biol Crystallogr |
Volume | 54 |
Issue | Pt 5 |
Pagination | 844-7 |
Date Published | 1998 Sep 1 |
ISSN | 0907-4449 |
Keywords | Carbohydrate Metabolism, Carbohydrate Sequence, Crystallization, Crystallography, X-Ray, Fabaceae, Lectins, Models, Molecular, Molecular Sequence Data, Plant Lectins, Plants, Medicinal, Protein Conformation, Substrate Specificity |
Abstract | The chitobiose specific Ulex europaeus lectin II crystallizes in space group P3221 with unit-cell dimensions a = b = 105.54, c = 176.26 A. The asymmetric unit contains a complete lectin tetramer. The crystals were shown to diffract to 4.5 A on a rotating-anode source and to 2.7 A at the Daresbury synchrotron source. Molecular replacement and subsequent rigid-body refinement using data to 4.5 A yielded a solution corresponding to a tetramer very similar to that of phytohemagglutinin-L and soybean agglutinin. The monomers in the Ulex lectin tetramer are rotated approximately 5 degrees compared with the phytohemagglutinin-L and soybean agglutinin structures. |
Alternate Journal | Acta Crystallogr. D Biol. Crystallogr. |
PubMed ID | 9757099 |
- Log in to post comments
- Google Scholar
- PubMed