Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs.

TitleHelicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs.
Publication TypeJournal Article
Year of Publication2016
AuthorsJavaheri, A., T. Kruse, K. Moonens, R. Mejías-Luque, A. Debraekeleer, C. I. Asche, N. Tegtmeyer, B. Kalali, N. C. Bach, S. A. Sieber, D. J. Hill, V. Königer, C. R. Hauck, R. Moskalenko, R. Haas, D. H. Busch, E. Klaile, H. Slevogt, A. Schmidt, S. Backert, H. Remaut, B. B. Singer, and M. Gerhard
JournalNat Microbiol
Volume2
Pagination16189
Date Published2016 Oct 17
ISSN2058-5276
KeywordsAdhesins, Bacterial, Antigens, Bacterial, Bacterial Adhesion, Bacterial Proteins, Cell Adhesion Molecules, Cell Line, Crystallography, X-Ray, Helicobacter pylori, Host-Pathogen Interactions, Humans, Interleukin-8, Protein Binding, Protein Conformation, Protein Transport, Virulence
Abstract

Helicobacter pylori specifically colonizes the human gastric epithelium and is the major causative agent for ulcer disease and gastric cancer development. Here, we identify members of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family as receptors of H. pylori and show that HopQ is the surface-exposed adhesin that specifically binds human CEACAM1, CEACAM3, CEACAM5 and CEACAM6. HopQ-CEACAM binding is glycan-independent and targeted to the N-domain. H. pylori binding induces CEACAM1-mediated signalling, and the HopQ-CEACAM1 interaction enables translocation of the virulence factor CagA into host cells and enhances the release of pro-inflammatory mediators such as interleukin-8. Based on the crystal structure of HopQ, we found that a β-hairpin insertion (HopQ-ID) in HopQ's extracellular 3+4 helix bundle domain is important for CEACAM binding. A peptide derived from this domain competitively inhibits HopQ-mediated activation of the Cag virulence pathway, as genetic or antibody-mediated abrogation of the HopQ function shows. Together, our data suggest the HopQ-CEACAM1 interaction to be a potentially promising novel therapeutic target to combat H. pylori-associated diseases.

DOI10.1038/nmicrobiol.2016.189
Alternate JournalNat Microbiol
PubMed ID27748768
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