Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody.

TitleCrystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody.
Publication TypeJournal Article
Year of Publication2016
AuthorsCho, K. Joon, B. Schepens, K. Moonens, L. Deng, W. Fiers, H. Remaut, and X. Saelens
JournalJ Virol
Volume90
Issue1
Pagination611-5
Date Published2016 01 01
ISSN1098-5514
KeywordsAnimals, Antibodies, Monoclonal, Antibodies, Viral, Cell Line, Crystallography, X-Ray, Humans, Mice, Inbred BALB C, Protein Binding, Protein Conformation, Viral Matrix Proteins
Abstract

We report the crystal structure of the M2 ectodomain (M2e) in complex with a monoclonal antibody that binds the amino terminus of M2. M2e extends into the antibody binding site to form an N-terminal β-turn near the bottom of the paratope. This M2e folding differs significantly from that of M2e in complex with an antibody that binds another part of M2e. This suggests that M2e can adopt at least two conformations that can elicit protective antibodies.

DOI10.1128/JVI.02105-15
Alternate JournalJ. Virol.
PubMed ID26468526
PubMed Central IDPMC4702545
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