Experimental African Trypanosome Infection by Needle Passage or Natural Tsetse Fly Challenge Thwarts the Development of Collagen-Induced Arthritis in DBA/1 Prone Mice via an Impairment of Antigen Specific B Cell Autoantibody Titers.

TitleExperimental African Trypanosome Infection by Needle Passage or Natural Tsetse Fly Challenge Thwarts the Development of Collagen-Induced Arthritis in DBA/1 Prone Mice via an Impairment of Antigen Specific B Cell Autoantibody Titers.
Publication TypeJournal Article
Year of Publication2015
AuthorsDe Trez, C., B. Katsandegwaza, G. Caljon, and S. Magez
JournalPLoS One
Volume10
Issue6
Paginatione0130431
Date Published2015
ISSN1932-6203
KeywordsAnimals, Antigens, Arthritis, Experimental, B-Lymphocytes, Humans, Mice, Mice, Inbred DBA, Needles, Trypanosoma brucei brucei, Trypanosoma brucei gambiense, Trypanosoma congolense, Trypanosomiasis, African, Tsetse Flies
Abstract

Collagen-induced arthritis is a B cell-mediated autoimmune disease. Recently published studies have demonstrated that in some rare cases pathogens can confer protection from autoimmunity. Trypanosoma brucei parasites are tsetse fly transmitted extracellular protozoans causing sleeping sickness disease in humans and Nagana in livestock in sub-Saharan endemic areas. In the past, we demonstrated that trypanosome infections impair B cell homeostasis and abolish vaccine-induced protection against unrelated antigens. Hence, here we hypothesized that trypanosome infection can affect the onset of CIA by specifically dampening specific B-cell responses and type II collagen antibody titers in DBA/1 prone mice. We observed a substantial delay in the onset of collagen-induced arthritis in T. brucei-infected DBA/1 mice that correlates with a drastic decrease of type II collagen titers of the different IgG isotypes in the serum. Treatment of infected mice with Berenil, a trypanocidal drug, restored the development of CIA-associated clinical symptoms. Interestingly, these data were confirmed by the challenge of immunized DBA/1 prone mice with T. brucei-infected tsetse flies. Together, these results demonstrate that T. brucei infection is impairing the maintenance of the antigen specific plasma B cell pool driving the development of CIA in DBA/1 prone mice.

DOI10.1371/journal.pone.0130431
Alternate JournalPLoS ONE
PubMed ID26110416
PubMed Central IDPMC4482398
Research group: