|Title||Nanobodies to Study G Protein-Coupled Receptor Structure and Function.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Manglik, A., B. K. Kobilka, and J. Steyaert|
|Journal||Annu Rev Pharmacol Toxicol|
|Date Published||2017 01 06|
|Keywords||Animals, Dose-Response Relationship, Drug, Humans, Isoproterenol, Ligands, Protein Binding, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Receptors, G-Protein-Coupled, Single-Domain Antibodies|
Ligand-induced activation of G protein-coupled receptors (GPCRs) is a key mechanism permitting communication between cells and organs. Enormous progress has recently elucidated the structural and dynamic features of GPCR transmembrane signaling. Nanobodies, the recombinant antigen-binding fragments of camelid heavy-chain-only antibodies, have emerged as important research tools to lock GPCRs in particular conformational states. Active-state stabilizing nanobodies have elucidated several agonist-bound structures of hormone-activated GPCRs and have provided insight into the dynamic character of receptors. Nanobodies have also been used to stabilize transient GPCR transmembrane signaling complexes, yielding the first structural insights into GPCR signal transduction across the cellular membrane. Beyond their in vitro uses, nanobodies have served as conformational biosensors in living systems and have provided novel ways to modulate GPCR function. Here, we highlight several examples of how nanobodies have enabled the study of GPCR function and give insights into potential future uses of these important tools.
|Alternate Journal||Annu. Rev. Pharmacol. Toxicol.|
|PubMed Central ID||PMC5500200|
|Grant List||R01 GM083118 / GM / NIGMS NIH HHS / United States |
R01 NS028471 / NS / NINDS NIH HHS / United States
T32 GM008294 / GM / NIGMS NIH HHS / United States
Nanobodies to Study G Protein-Coupled Receptor Structure and Function.