Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.

TitlePurification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.
Publication TypeJournal Article
Year of Publication1993
AuthorsSteyaert, J., L. Van Melderen, P. Bernard, M. H. Thi, R. Loris, L. Wyns, and M. Couturier
JournalJ Mol Biol
Volume231
Issue2
Pagination513-5
Date Published1993 May 20
ISSN0022-2836
KeywordsBacterial Proteins, Bacterial Toxins, Circular Dichroism, Crystallization, Escherichia coli, F Factor, Topoisomerase I Inhibitors, X-Ray Diffraction
Abstract

Large crystals of the Escherichia coli F plasmid CcdB killer protein were grown from solutions containing 32% ammonium sulphate. The crystals belong to space group P4(2)2(1)2 with a = b = 104.52 A and c = 88.45 A or P2(1)2(1)2(1) with a = 77.62 A, b = 93.28 A and c = 141.44 A. Both crystal forms diffract to 2.6 A resolution. Structure determination by multiple isomorphous replacement is under way.

DOI10.1006/jmbi.1993.1301
Alternate JournalJ. Mol. Biol.
PubMed ID8389886