Purification, crystallization, and preliminary X-ray studies on the rhizome lectin from stinging nettle and its complex with NN'N"-triacetylchitotriose.

TitlePurification, crystallization, and preliminary X-ray studies on the rhizome lectin from stinging nettle and its complex with NN'N"-triacetylchitotriose.
Publication TypeJournal Article
Year of Publication1993
AuthorsLoris, R., M. H. Thi, J. Lisgarten, and L. Wyns
JournalProteins
Volume15
Issue2
Pagination205-8
Date Published1993 Feb
ISSN0887-3585
KeywordsBinding Sites, Crystallization, Lectins, Molecular Structure, Plant Lectins, Plants, Trisaccharides, X-Ray Diffraction
Abstract

Single crystals were grown from affinity-purified stinging nettle lectin and from its complex with the specific trisaccharide NN'N"-triacetylchitotriose by vapor diffusion at room temperature. The lectin crystallizes in space group P2(1)2(1)2(1) with unit cell dimensions a = 54.3 (1) A, b = 62.2 (1) A, and c = 92.4 (2) A, and diffracts to 3.0 A resolution. The asymmetric unit contains three lectin monomers. The crystals of the lectin-trisaccharide complex have space group P2(1)2(1)2(1) with cell constants a = 37.69 (4) A, b = 48.97 (6) A, and c = 57.32 (4) A. These crystals diffract to at least 2.0 A resolution and the asymmetric unit contains one lectin monomer. A three-dimensional X-ray structure determination is on its way.

DOI10.1002/prot.340150210
Alternate JournalProteins
PubMed ID8441755