Title | Purification, crystallization, and preliminary X-ray studies on the rhizome lectin from stinging nettle and its complex with NN'N"-triacetylchitotriose. |
Publication Type | Journal Article |
Year of Publication | 1993 |
Authors | Loris, R., M. H. Thi, J. Lisgarten, and L. Wyns |
Journal | Proteins |
Volume | 15 |
Issue | 2 |
Pagination | 205-8 |
Date Published | 1993 Feb |
ISSN | 0887-3585 |
Keywords | Binding Sites, Crystallization, Lectins, Molecular Structure, Plant Lectins, Plants, Trisaccharides, X-Ray Diffraction |
Abstract | Single crystals were grown from affinity-purified stinging nettle lectin and from its complex with the specific trisaccharide NN'N"-triacetylchitotriose by vapor diffusion at room temperature. The lectin crystallizes in space group P2(1)2(1)2(1) with unit cell dimensions a = 54.3 (1) A, b = 62.2 (1) A, and c = 92.4 (2) A, and diffracts to 3.0 A resolution. The asymmetric unit contains three lectin monomers. The crystals of the lectin-trisaccharide complex have space group P2(1)2(1)2(1) with cell constants a = 37.69 (4) A, b = 48.97 (6) A, and c = 57.32 (4) A. These crystals diffract to at least 2.0 A resolution and the asymmetric unit contains one lectin monomer. A three-dimensional X-ray structure determination is on its way. |
DOI | 10.1002/prot.340150210 |
Alternate Journal | Proteins |
PubMed ID | 8441755 |
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