|Title||An essential thioredoxin is involved in the control of the cell cycle in the bacterium .|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Goemans, C. V., F. Beaufay, K. Wahni, I. Van Molle, J. Messens, and J-F. Collet|
|Journal||J Biol Chem|
|Date Published||2018 Mar 09|
Thioredoxins (Trxs) are antioxidant proteins that are conserved among all species. These proteins have been extensively studied and perform reducing reactions on a broad range of substrates. Here, we identified Trx1 (CCNA_03653; Trx1) as an oxidoreductase that is involved in the cell cycle progression of this model bacterium and is required to sustain life. Intriguingly, the abundance of Trx1 varies throughout the cell cycle: although the expression of Trx1 is induced in stalked cells, right before DNA replication initiation, Trx1 is actively degraded by the ClpXP protease in predivisional cells. Importantly, we demonstrated that regulation of the abundance of Trx1 is crucial for cell growth and survival as modulating Trx1 levels leads to cell death. Finally, we also report a comprehensive biochemical and structural characterization of this unique and essential Trx. The requirement to precisely control the abundance of Trx1 for cell survival underlines the importance of redox control for optimal cell cycle progression in .
|Alternate Journal||J. Biol. Chem.|
|PubMed Central ID||PMC5846133|
An essential thioredoxin is involved in the control of the cell cycle in the bacterium .