|Title||Co-Evolution of Intrinsically Disordered Proteins with Folded Partners Witnessed by Evolutionary Couplings.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Pancsa, R., F. Zsolyomi, and P. Tompa|
|Journal||Int J Mol Sci|
|Date Published||2018 Oct 25|
|Keywords||Animals, Evolution, Molecular, Humans, Intrinsically Disordered Proteins, Protein Binding, Protein Folding|
Although improved strategies for the detection and analysis of evolutionary couplings (ECs) between protein residues already enable the prediction of protein structures and interactions, they are mostly restricted to conserved and well-folded proteins. Whereas intrinsically disordered proteins (IDPs) are central to cellular interaction networks, due to the lack of strict structural constraints, they undergo faster evolutionary changes than folded domains. This makes the reliable identification and alignment of IDP homologs difficult, which led to IDPs being omitted in most large-scale residue co-variation analyses. By preforming a dedicated analysis of phylogenetically widespread bacterial IDP⁻partner interactions, here we demonstrate that partner binding imposes constraints on IDP sequences that manifest in detectable interprotein ECs. These ECs were not detected for interactions mediated by short motifs, rather for those with larger IDP⁻partner interfaces. Most identified coupled residue pairs reside close (<10 Å) to each other on the interface, with a third of them forming multiple direct atomic contacts. EC-carrying interfaces of IDPs are enriched in negatively charged residues, and the EC residues of both IDPs and partners preferentially reside in helices. Our analysis brings hope that IDP⁻partner interactions difficult to study could soon be successfully dissected through residue co-variation analysis.
|Alternate Journal||Int J Mol Sci|
|PubMed Central ID||PMC6274761|
|Grant List||ALTF 702-2015 / / European Molecular Biology Organization / |
Premium_2017-48 / / Magyar Tudományos Akadémia /
G.0029.12 / / Fonds Wetenschappelijk Onderzoek /
Co-Evolution of Intrinsically Disordered Proteins with Folded Partners Witnessed by Evolutionary Couplings.