Co-Evolution of Intrinsically Disordered Proteins with Folded Partners Witnessed by Evolutionary Couplings.

TitleCo-Evolution of Intrinsically Disordered Proteins with Folded Partners Witnessed by Evolutionary Couplings.
Publication TypeJournal Article
Year of Publication2018
AuthorsPancsa, R., F. Zsolyomi, and P. Tompa
JournalInt J Mol Sci
Volume19
Issue11
Date Published2018 Oct 25
ISSN1422-0067
KeywordsAnimals, Evolution, Molecular, Humans, Intrinsically Disordered Proteins, Protein Binding, Protein Folding
Abstract

Although improved strategies for the detection and analysis of evolutionary couplings (ECs) between protein residues already enable the prediction of protein structures and interactions, they are mostly restricted to conserved and well-folded proteins. Whereas intrinsically disordered proteins (IDPs) are central to cellular interaction networks, due to the lack of strict structural constraints, they undergo faster evolutionary changes than folded domains. This makes the reliable identification and alignment of IDP homologs difficult, which led to IDPs being omitted in most large-scale residue co-variation analyses. By preforming a dedicated analysis of phylogenetically widespread bacterial IDP⁻partner interactions, here we demonstrate that partner binding imposes constraints on IDP sequences that manifest in detectable interprotein ECs. These ECs were not detected for interactions mediated by short motifs, rather for those with larger IDP⁻partner interfaces. Most identified coupled residue pairs reside close (<10 Å) to each other on the interface, with a third of them forming multiple direct atomic contacts. EC-carrying interfaces of IDPs are enriched in negatively charged residues, and the EC residues of both IDPs and partners preferentially reside in helices. Our analysis brings hope that IDP⁻partner interactions difficult to study could soon be successfully dissected through residue co-variation analysis.

DOI10.3390/ijms19113315
Alternate JournalInt J Mol Sci
PubMed ID30366362
PubMed Central IDPMC6274761
Grant ListALTF 702-2015 / / European Molecular Biology Organization /
Premium_2017-48 / / Magyar Tudományos Akadémia /
G.0029.12 / / Fonds Wetenschappelijk Onderzoek /
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