H, C, and N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family.

TitleH, C, and N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family.
Publication TypeJournal Article
Year of Publication2019
AuthorsProlič-Kalinšek, M., P. De Bruyn, D. Jurenas, L. Van Melderen, R. Loris, and A. N. Volkov
JournalBiomol NMR Assign
Date Published2019 Oct 17
ISSN1874-270X
Abstract

The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the H, N and C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.

DOI10.1007/s12104-019-09915-9
Alternate JournalBiomol NMR Assign
PubMed ID31625047
Grant ListG.0226.17N / / Fonds Wetenschappelijk Onderzoek /
SPR13 / / Vrije Universiteit Brussel /
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