The quiescin sulfhydryl oxidase (hQSOX1b) tunes the expression of resistin-like molecule alpha (RELM-α or mFIZZ1) in a wheat germ cell-free extract.

TitleThe quiescin sulfhydryl oxidase (hQSOX1b) tunes the expression of resistin-like molecule alpha (RELM-α or mFIZZ1) in a wheat germ cell-free extract.
Publication TypeJournal Article
Year of Publication2013
AuthorsGad, W., M. G. Nair, K. Van Belle, K. Wahni, H. De Greve, J. A. Van Ginderachter, G. Vandenbussche, Y. Endo, D. Artis, and J. Messens
JournalPLoS One
Volume8
Issue1
Paginatione55621
Date Published2013
ISSN1932-6203
KeywordsAmino Acid Sequence, Animals, Disulfides, Escherichia coli, Gene Expression Regulation, Germ Cells, Humans, Intercellular Signaling Peptides and Proteins, Mice, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases Acting on Sulfur Group Donors, Protein Folding, Protein Structure, Secondary, Sequence Alignment, Solubility, Triticum
Abstract

BACKGROUND: Although disulfide bond formation in proteins is one of the most common types of post-translational modifications, the production of recombinant disulfide-rich proteins remains a challenge. The most popular host for recombinant protein production is Escherichia coli, but disulfide-rich proteins are here often misfolded, degraded, or found in inclusion bodies.METHODOLOGY/PRINCIPAL FINDINGS: We optimize an in vitro wheat germ translation system for the expression of an immunological important eukaryotic protein that has to form five disulfide bonds, resistin-like alpha (mFIZZ1). Expression in combination with human quiescin sulfhydryl oxidase (hQSOX1b), the disulfide bond-forming enzyme of the endoplasmic reticulum, results in soluble, intramolecular disulfide bonded, monomeric, and biological active protein. The mFIZZ1 protein clearly suppresses the production of the cytokines IL-5 and IL-13 in mouse splenocytes cultured under Th2 permissive conditions.CONCLUSION/SIGNIFICANCE: The quiescin sulfhydryl oxidase hQSOX1b seems to function as a chaperone and oxidase during the oxidative folding. This example for mFIZZ1 should encourage the design of an appropriate thiol/disulfide oxidoreductase-tuned cell free expression system for other challenging disulfide rich proteins.

DOI10.1371/journal.pone.0055621
Alternate JournalPLoS ONE
PubMed ID23383248
PubMed Central IDPMC3561318
Grant ListR01 AI091759 / AI / NIAID NIH HHS / United States
AI061570 / AI / NIAID NIH HHS / United States
AI074878 / AI / NIAID NIH HHS / United States
AI091759 / AI / NIAID NIH HHS / United States
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