|Title||Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Van der Henst, C., A. Sophie Vanhove, N. Carolina D. Dörr, S. Stutzmann, C. Stoudmann, S. Clerc, T. Scrignari, C. Maclachlan, G. Knott, and M. Blokesch|
|Date Published||2018 08 27|
|Keywords||Acanthamoeba castellanii, Analysis of Variance, Ecosystem, Genetic Engineering, Host-Pathogen Interactions, Microscopy, Confocal, Microscopy, Electron, Transmission, Reverse Transcriptase Polymerase Chain Reaction, Vibrio cholerae, Virulence|
Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonly found in aquatic habitats. Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with Acanthamoeba castellanii being one of the best-studied aquatic amoebae. We previously showed that V. cholerae resists digestion by A. castellanii and establishes a replication niche within the host's osmoregulatory organelle. In this study, we decipher the molecular mechanisms involved in the maintenance of V. cholerae's intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrate that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, have a key role in the replication and transmission of V. cholerae in its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the species V. cholerae.
|Alternate Journal||Nat Commun|
|PubMed Central ID||PMC6110790|
|Grant List||309064 / / European Research Council / International |
309064 / / EC | European Research Council (ERC) / International
724630 / / EC | European Research Council (ERC) / International
55008726 / / Howard Hughes Medical Institute (HHMI) / International
Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions.