Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY.

TitleIndependent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY.
Publication TypeJournal Article
Year of Publication2016
AuthorsMetzger, L. C., S. Stutzmann, T. Scrignari, C. Van der Henst, N. Matthey, and M. Blokesch
JournalCell Rep
Volume15
Issue5
Pagination951-958
Date Published2016 05 03
ISSN2211-1247
KeywordsBacterial Proteins, Bacterial Secretion Systems, Cyclic GMP, Gene Expression Regulation, Bacterial, Models, Biological, Sequence Homology, Amino Acid, Vibrio cholerae
Abstract

Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the natural competence regulon in V. cholerae and is activated by the regulator TfoX. Here, we identify the TfoX homolog TfoY as a second activator of the T6SS. Importantly, despite inducing the same T6SS core machinery, the overall regulons differ significantly for TfoX and TfoY. We show that TfoY does not contribute to competence induction. Instead, TfoY drives the production of T6SS-dependent and T6SS-independent toxins, together with an increased motility phenotype. Hence, we conclude that V. cholerae uses its sole T6SS in response to diverse cues and for distinctive outcomes: either to kill for the prey's DNA, leading to horizontal gene transfer, or as part of a defensive escape reaction.

DOI10.1016/j.celrep.2016.03.092
Alternate JournalCell Rep
PubMed ID27117415
PubMed Central IDPMC4858559
Grant List309064 / / European Research Council / International
Research group: