Polar growth in the Alphaproteobacterial order Rhizobiales.

TitlePolar growth in the Alphaproteobacterial order Rhizobiales.
Publication TypeJournal Article
Year of Publication2012
AuthorsBrown, P. J. B., M. A. de Pedro, D. T. Kysela, C. Van der Henst, J. Kim, X. De Bolle, C. Fuqua, and Y. V. Brun
JournalProc Natl Acad Sci U S A
Volume109
Issue5
Pagination1697-701
Date Published2012 Jan 31
ISSN1091-6490
KeywordsAlphaproteobacteria, Phylogeny, Rhizobiaceae
Abstract

Elongation of many rod-shaped bacteria occurs by peptidoglycan synthesis at discrete foci along the sidewall of the cells. However, within the Rhizobiales, there are many budding bacteria, in which new cell growth is constrained to a specific region. The phylogeny of the Rhizobiales indicates that this mode of zonal growth may be ancestral. We demonstrate that the rod-shaped bacterium Agrobacterium tumefaciens grows unidirectionally from the new pole generated after cell division and has an atypical peptidoglycan composition. Polar growth occurs under all conditions tested, including when cells are attached to a plant root and under conditions that induce virulence. Finally, we show that polar growth also occurs in the closely related bacteria Sinorhizobium meliloti, Brucella abortus, and Ochrobactrum anthropi. We find that unipolar growth is an ancestral and conserved trait among the Rhizobiales, which includes important mutualists and pathogens of plants and animals.

DOI10.1073/pnas.1114476109
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID22307633
PubMed Central IDPMC3277149
Grant ListGM080546 / GM / NIGMS NIH HHS / United States
AI072992 / AI / NIAID NIH HHS / United States
R01 GM051986 / GM / NIGMS NIH HHS / United States
F32 AI072992 / AI / NIAID NIH HHS / United States
R01 GM077648 / GM / NIGMS NIH HHS / United States
F32 GM083581 / GM / NIGMS NIH HHS / United States
R01 GM080546 / GM / NIGMS NIH HHS / United States
GM051986 / GM / NIGMS NIH HHS / United States
GM083581 / GM / NIGMS NIH HHS / United States
GM077648 / GM / NIGMS NIH HHS / United States
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