Structural and kinetic characterization of Trypanosoma congolense pyruvate kinase.

TitleStructural and kinetic characterization of Trypanosoma congolense pyruvate kinase.
Publication TypeJournal Article
Year of Publication2020
AuthorsTorres, J. Esteban Pi, M. Yuan, J. Goossens, W. Versées, G. Caljon, P. A. Michels, M. D. Walkinshaw, S. Magez, and Y. G. - J. Sterckx
JournalMol Biochem Parasitol
Volume236
Pagination111263
Date Published2020 Mar
ISSN1872-9428
Abstract

Trypanosoma are blood-borne parasites and are the causative agents of neglected tropical diseases (NTDs) affecting both humans and animals. These parasites mainly rely on glycolysis for their energy production within the mammalian host, which is why trypanosomal glycolytic enzymes have been pursued as interesting targets for the development of trypanocidal drugs. The structure-function relationships of pyruvate kinases (PYKs) from trypanosomatids (Trypanosoma and Leishmania) have been well-studied within this context. In this paper, we describe the structural and enzymatic characterization of PYK from T. congolense (TcoPYK), the main causative agent of Animal African Trypanosomosis (AAT), by employing a combination of enzymatic assays, thermal unfolding studies and X-ray crystallography.

DOI10.1016/j.molbiopara.2020.111263
Alternate JournalMol. Biochem. Parasitol.
PubMed ID32084384
subject_category: 
Research group: