The thermodynamics of thiol sulfenylation.

TitleThe thermodynamics of thiol sulfenylation.
Publication TypeJournal Article
Year of Publication2012
AuthorsBilliet, L., P. Geerlings, J. Messens, and G. Roos
JournalFree Radic Biol Med
Volume52
Issue8
Pagination1473-85
Date Published2012 Apr 15
Type of Articleredox
ISSN1873-4596
KeywordsAmino Acid Sequence, Models, Molecular, Molecular Sequence Data, Sequence Homology, Amino Acid, Sulfamerazine, Sulfhydryl Compounds, Thermodynamics
Abstract

Protein sulfenic acids are essential cysteine oxidations in cellular signaling pathways. The thermodynamics that drive protein sulfenylation are not entirely clear. Experimentally, sulfenic acid reduction potentials are hard to measure, because of their highly reactive nature. We designed a calculation method, the reduction potentials from electronic energies (REE) method, to give for the first time insight into the thermodynamic aspects of protein sulfenylation. The REE method is based on the correlation between reaction path-independent reaction energies and free energies of a series of analogous reactions. For human peroxiredoxin (Tpx-B), an antioxidant enzyme that forms a sulfenic acid on one of its active-site cysteines during reactive oxygen scavenging, we found that the reduction potential depends on the composition of the active site and on the protonation state of the cysteine. Interaction with polar residues directs the RSO(-)/RS(-) reduction to a lower potential than the RSOH/RSH reduction. A conserved arginine that thermodynamically favors the sulfenylation reaction might be a good candidate to favor the reaction kinetics. The REE method is not limited to thiol sulfenylation, but can be broadly applied to understand protein redox biology in general.

DOI10.1016/j.freeradbiomed.2011.12.029
Alternate JournalFree Radic. Biol. Med.
PubMed ID22326773
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