Synthesis of glial fibrillary acidic protein in rat C6 glioma in chemically defined medium: cyclic AMP-dependent transcriptional and translational regulation.

TitleSynthesis of glial fibrillary acidic protein in rat C6 glioma in chemically defined medium: cyclic AMP-dependent transcriptional and translational regulation.
Publication TypeJournal Article
Year of Publication1992
AuthorsMessens, J., and H. Slegers
JournalJ Neurochem
Volume58
Issue6
Pagination2071-80
Date Published1992 Jun
ISSN0022-3042
KeywordsAnimals, Bucladesine, Culture Media, Cyclic AMP, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Neoplastic, Glial Fibrillary Acidic Protein, Glioma, Lipopolysaccharides, Protein Biosynthesis, Rats, RNA, Messenger, Transcription, Genetic, Tumor Cells, Cultured, Vimentin
Abstract

Glial fibrillary acidic protein (GFA) expression was induced in rat C6 glioma in chemically defined medium by the addition of N6, O2'-dibutyryl cyclic AMP (dbcAMP). Induction was dependent on the increase in intracellular cyclic AMP (cAMP), which was linearly correlated with added dbcAMP. Contrary to GFA mRNA synthesis, which can be obtained by cAMP-dependent and -independent pathways, translation of mRNA into GFA was observed only above a cellular cAMP concentration of approximately 0.2 fmol/cell. dbcAMP stimulation did not affect the vimentin concentration, which remained at a low level, but changed the cellular morphology from a bipolar to a stellate shape. A similar morphological change was observed after stimulation of C6 with lipopolysaccharide (LPS). However, LPS did not significantly increase the intracellular concentration of cAMP and the LPS-induced mRNA was not translated into GFA. Our results indicate that GFA synthesis is regulated at the mRNA level and at the translational level and that a cAMP-dependent mechanism determines the ultimate synthesis of GFA by a yet unknown mechanism.

Alternate JournalJ. Neurochem.
PubMed ID1315374
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