Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity.

TitleTsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity.
Publication TypeJournal Article
Year of Publication2012
AuthorsCaljon, G., K. De Ridder, B. Stijlemans, M. Coosemans, S. Magez, P. De Baetselier, and J. Van Den Abbeele
JournalPLoS One
Volume7
Issue10
Paginatione47233
Date Published2012
Type of Articleparasites
ISSN1932-6203
KeywordsAnimals, Endonucleases, Expressed Sequence Tags, Hydrogen-Ion Concentration, Insect Proteins, Salivary Glands, Salivary Proteins and Peptides, Tsetse Flies
Abstract

Analysis of the tsetse fly salivary gland EST database revealed the presence of a highly enriched cluster of putative endonuclease genes, including tsal1 and tsal2. Tsal proteins are the major components of tsetse fly (G. morsitans morsitans) saliva where they are present as monomers as well as high molecular weight complexes with other saliva proteins. We demonstrate that the recombinant tsetse salivary gland proteins 1&2 (Tsal1&2) display DNA/RNA non-specific, high affinity nucleic acid binding with K(D) values in the low nanomolar range and a non-exclusive preference for duplex. These Tsal proteins exert only a residual nuclease activity with a preference for dsDNA in a broad pH range. Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents.

DOI10.1371/journal.pone.0047233
Alternate JournalPLoS ONE
PubMed ID23110062
PubMed Central IDPMC3479092
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