iNOS-producing inflammatory dendritic cells constitute the major infected cell type during the chronic Leishmania major infection phase of C57BL/6 resistant mice.

TitleiNOS-producing inflammatory dendritic cells constitute the major infected cell type during the chronic Leishmania major infection phase of C57BL/6 resistant mice.
Publication TypeJournal Article
Year of Publication2009
AuthorsDe Trez, C., Magez S., Akira S., Ryffel B., Carlier Y., and Muraille E.
JournalPLoS Pathog
Volume5
Issue6
Paginatione1000494
Date Published2009 Jun
ISSN1553-7374
KeywordsAnimals, Chronic Disease, Cytokines, Dendritic Cells, Flow Cytometry, Inflammation, Leishmania major, Leishmaniasis, Cutaneous, Luminescent Proteins, Lymph Nodes, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Nitric Oxide Synthase Type II, Phenotype, Receptors, CCR2, Statistics, Nonparametric
Abstract

Leishmania major parasites reside and multiply in late endosomal compartments of host phagocytic cells. Immune control of Leishmania growth absolutely requires expression of inducible Nitric Oxide Synthase (iNOS/NOS2) and subsequent production of NO. Here, we show that CD11b+ CD11c+ Ly-6C+ MHC-II+ cells are the main iNOS-producing cells in the footpad lesion and in the draining lymph node of Leishmania major-infected C57BL/6 mice. These cells are phenotypically similar to iNOS-producing inflammatory DC (iNOS-DC) observed in the mouse models of Listeria monocytogenes and Brucella melitensis infection. The use of DsRed-expressing parasites demonstrated that these iNOS-producing cells are the major infected population in the lesions and the draining lymph nodes. Analysis of various genetically deficient mouse strains revealed the requirement of CCR2 expression for the recruitment of iNOS-DC in the draining lymph nodes, whereas their activation is strongly dependent on CD40, IL-12, IFN-gamma and MyD88 molecules with a partial contribution of TNF-alpha and TLR9. In contrast, STAT-6 deficiency enhanced iNOS-DC recruitment and activation in susceptible BALB/c mice, demonstrating a key role for IL-4 and IL-13 as negative regulators. Taken together, our results suggest that iNOS-DC represent a major class of Th1-regulated effector cell population and constitute the most frequent infected cell type during chronic Leishmania major infection phase of C57BL/6 resistant mice.

DOI10.1371/journal.ppat.1000494
Alternate JournalPLoS Pathog.
PubMed ID19557162
PubMed Central IDPMC2695779
Research group: