Identification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes.

TitleIdentification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes.
Publication TypeJournal Article
Year of Publication2007
AuthorsSemballa, S., M. C. Okomo-Assoumou, P. Holzmuller, P. B├╝scher, S. Magez, J. L. Lemesre, S. Daulouede, P. Courtois, M. Geffard, and P. Vincendeau
JournalExp Parasitol
Volume115
Issue2
Pagination173-80
Date Published2007 Feb
Type of Articleparasites
ISSN0014-4894
KeywordsAnimals, Antibodies, Protozoan, Blotting, Western, Chromatography, Affinity, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Epitopes, Female, Humans, Mice, Rabbits, Trypanosoma brucei brucei, Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosomiasis, African, Variant Surface Glycoproteins, Trypanosoma
Abstract

Antibodies (Ab) directed against a tryptophan-like epitope (WE) were previously detected in patients with human African trypanosomiasis (HAT). We investigated whether or not these Ab resulted from immunization against trypanosome antigen(s) expressing a WE. By Western blotting, we identified an antigen having an apparent molecular weight ranging from 60 to 65 kDa, recognized by purified rabbit anti-WE Ab. This antigen, present in trypomastigote forms, was absent in procyclic forms and Trypanosoma cruzi trypomastigotes. Using purified variable surface glycoproteins (VSG) from various trypanosomes, we showed that VSG was the parasite antigen recognized by these rabbit Ab. Anti-WE and anti-VSG Ab were purified from HAT sera by affinity chromatography. Immunoreactivity of purified antibodies eluted from affinity columns and of depleted fractions showed that WE was one of the epitopes borne by VSG. These data underline the existence of an invariant WE in the structure of VSG from several species of African trypanosomes.

DOI10.1016/j.exppara.2006.08.008
Alternate JournalExp. Parasitol.
PubMed ID17014849