Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes.

TitleDirect detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes.
Publication TypeJournal Article
Year of Publication2002
AuthorsRadwanska, M., S. Magez, H. Perry-O'Keefe, H. Stender, J. Coull, J. M. Sternberg, P. B├╝scher, and J. J. Hyldig-Nielsen
JournalJ Clin Microbiol
Volume40
Issue11
Pagination4295-7
Date Published2002 Nov
ISSN0095-1137
KeywordsAnimals, Blood, Cattle, DNA, Ribosomal, Humans, In Situ Hybridization, Fluorescence, Nucleic Acid Probes, Peptide Nucleic Acids, RNA, Ribosomal, 18S, Trypanosoma, Trypanosomiasis, African
Abstract

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

Alternate JournalJ. Clin. Microbiol.
PubMed ID12409413
PubMed Central IDPMC139669