Murine tumour necrosis factor plays a protective role during the initial phase of the experimental infection with Trypanosoma brucei brucei.

TitleMurine tumour necrosis factor plays a protective role during the initial phase of the experimental infection with Trypanosoma brucei brucei.
Publication TypeJournal Article
Year of Publication1993
AuthorsMagez, S., R. Lucas, A. Darji, E. B. Songa, R. Hamers, and P. De Baetselier
JournalParasite Immunol
Volume15
Issue11
Pagination635-41
Date Published1993 Nov
ISSN0141-9838
KeywordsAnimals, Antibodies, Monoclonal, Cell Extracts, Cell Line, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Macrophages, Mice, Mice, Inbred C3H, Mice, Inbred CBA, Parasitemia, Trypanosoma brucei brucei, Trypanosomiasis, African, Tumor Necrosis Factor-alpha
Abstract

Soluble extracts from salivarian trypanosomes (Trypanosoma brucei brucei, T. evansi and T. congolense) were shown to be capable of inducing murine tumour necrosis factor (mTNF) secretion, both in vivo and in vitro, whereas the soluble extract of an intracellular trypanosome (T. cruzi) failed to do so. Furthermore, the role of mTNF during the initial phase of experimental infections with T. brucei was studied by treating infected mice with mTNF-inducing trypanosoma soluble extract and with neutralizing monoclonal anti-mTNF antibodies. Treatment of the infected animals with different doses of T. brucei soluble extract resulted in a lower first parasitaemia peak (low lysate dose) and in a longer survival time or in a nearly total inhibition of parasite development (high lysate dose). Cotreatment of the infected mice with both anti-mTNF antibodies and a high dose of soluble extract completely restored the parasite development in both trypanosusceptible C3H/He mice and trypanosubtolerant CBA Ca mice, indicating a protective role of mTNF during the parasitaemia. Collectively these results suggest a negative influence of mTNF on T. brucei development in vivo.

Alternate JournalParasite Immunol.
PubMed ID7877840