Identification of intrinsically disordered proteins by a special 2D electrophoresis.

TitleIdentification of intrinsically disordered proteins by a special 2D electrophoresis.
Publication TypeJournal Article
Year of Publication2012
AuthorsTantos, Á., and P. Tompa
JournalMethods Mol Biol
Volume896
Pagination215-22
Date Published2012
Type of Articleidp
ISSN1940-6029
KeywordsElectrophoresis, Gel, Two-Dimensional, Proteins
Abstract

Intrinsically disordered proteins (IDPs) lack a well-defined three-dimensional structure under physiological conditions. They constitute a significant fraction of various proteomes and have significant roles in key cellular processes. Here we report the development of a two-dimensional electrophoresis technique for their de novo recognition and characterization. This technique consists of the combination of native and 8 M urea electrophoresis of heat-treated proteins where IDPs are expected to run into the diagonal of the gel, whereas globular proteins either precipitate upon heat treatment or unfold and run off the diagonal in the second dimension.

DOI10.1007/978-1-4614-3704-8_13
Alternate JournalMethods Mol. Biol.
PubMed ID22821526