Wide-line NMR and protein hydration.

TitleWide-line NMR and protein hydration.
Publication TypeJournal Article
Year of Publication2012
AuthorsTompa, K., M. Bokor, and P. Tompa
JournalMethods Mol Biol
Date Published2012
Type of Articlenmr
KeywordsAlgorithms, Arabidopsis Proteins, Fourier Analysis, Freeze Drying, Humans, Nuclear Magnetic Resonance, Biomolecular, Quantum Theory, Reference Standards, Solvents, Ubiquitin, Water

In this chapter, the reader is introduced to the basics of wide-line NMR, with particular focus on the following: (1) basic theoretical and experimental NMR elements, necessary before switching the spectrometer and designing the experiment, (2) models/theories for the interpretation of measured data, (3) definition of wide-line NMR spectrometry, the description of the measurement and evaluation variants, useful hints for the novice, (4) advice on selecting the solvent, which is not a trivial task, (5) a note of warning that not all data are acceptable in spite of the statistical confidence. Finally, we wrap up the chapter with the results on two proteins (a globular and an intrinsically disordered).

Alternate JournalMethods Mol. Biol.
PubMed ID22760320
Grant List / / Wellcome Trust / United Kingdom