|Title||Mycoredoxin-1 is one of the missing links in the oxidative stress defence mechanism of Mycobacteria.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Van Laer, K., L. Buts, N. Foloppe, D. Vertommen, K. Van Belle, K. Wahni, G. Roos, L. Nilsson, L. M. Mateos, M. Rawat, N. A. J. van Nuland, and J. Messens|
|Date Published||2012 Nov|
|Type of Article||redox|
|Keywords||Cysteine, Disulfides, Gene Deletion, Glycopeptides, Inositol, Magnetic Resonance Spectroscopy, Models, Molecular, Mycobacterium smegmatis, Oxidation-Reduction, Oxidative Stress, Oxidoreductases, Protein Conformation|
To survive hostile conditions, the bacterial pathogen Mycobacterium tuberculosis produces millimolar concentrations of mycothiol as a redox buffer against oxidative stress. The reductases that couple the reducing power of mycothiol to redox active proteins in the cell are not known. We report a novel mycothiol-dependent reductase (mycoredoxin-1) with a CGYC catalytic motif. With mycoredoxin-1 and mycothiol deletion strains of Mycobacterium smegmatis, we show that mycoredoxin-1 and mycothiol are involved in the protection against oxidative stress. Mycoredoxin-1 acts as an oxidoreductase exclusively linked to the mycothiol electron transfer pathway and it can reduce S-mycothiolated mixed disulphides. Moreover, we solved the solution structures of oxidized and reduced mycoredoxin-1, revealing a thioredoxin fold with a putative mycothiol-binding site. With HSQC snapshots during electron transport, we visualize the reduction of oxidized mycoredoxin-1 as a function of time and find that mycoredoxin-1 gets S-mycothiolated on its N-terminal nucleophilic cysteine. Mycoredoxin-1 has a redox potential of -218 mV and hydrogen bonding with neighbouring residues lowers the pKa of its N-terminal nucleophilic cysteine. Determination of the oxidized and reduced structures of mycoredoxin-1, better understanding of mycothiol-dependent reactions in general, will likely give new insights in how M. tuberculosis survives oxidative stress in human macrophages.
|Alternate Journal||Mol. Microbiol.|
|Grant List||GM061223 / GM / NIGMS NIH HHS / United States|
Mycoredoxin-1 is one of the missing links in the oxidative stress defence mechanism of Mycobacteria.