Title | H-start for exclusively heteronuclear NMR spectroscopy: the case of intrinsically disordered proteins. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Bermel, W., I. Bertini, V. Csizmok, I. C. Felli, R. Pierattelli, and P. Tompa |
Journal | J Magn Reson |
Volume | 198 |
Issue | 2 |
Pagination | 275-81 |
Date Published | 2009 Jun |
ISSN | 1096-0856 |
Keywords | Algorithms, Carbon Isotopes, Humans, Hydrogen, Neoplasm Proteins, Nitrogen Radioisotopes, Nuclear Magnetic Resonance, Biomolecular, Protein Carbonylation, Proteins |
Abstract | Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on 13C direct detection, which exploit the (1)H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa. |
DOI | 10.1016/j.jmr.2009.02.012 |
Alternate Journal | J. Magn. Reson. |
PubMed ID | 19307141 |
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