Calpain as a multi-site regulator of cell cycle.

TitleCalpain as a multi-site regulator of cell cycle.
Publication TypeJournal Article
Year of Publication2004
AuthorsJánossy, J., P. Ubezio, A. Apáti, M. Magócsi, P. Tompa, and P. Friedrich
JournalBiochem Pharmacol
Volume67
Issue8
Pagination1513-21
Date Published2004 Apr 15
ISSN0006-2952
KeywordsCalpain, Cell Cycle, Cell Division, Cell Survival, Flow Cytometry, G1 Phase, G2 Phase, Glycoproteins, Granulocyte-Macrophage Colony-Stimulating Factor, Humans, Mitosis, S Phase, Tumor Cells, Cultured
Abstract

Calpain has long been implicated in the regulation of cell cycle, mostly based on studies with inhibitors that lack strict specificity toward the enzyme. Further, previous work has primarily focused on one particular point, the G(1) checkpoint, and made no attempt at dissecting the full cycle in terms of calpain action. To extend and complement these findings, we tested the effect of a specific inhibitor, PD 150606, on granulocyte-macrophage-colony stimulating factor (GM-CSF)-stimulated human TF-1 cells by flow cytometry following single- and double labelling by propidium iodide and bromodeoxyuridine. Using a new algorithm of analysis, we determined the time-dependence of the absolute number of cells leaving G(1), S and G(2)M phases following the application of the inhibitor. Our results point to the simultaneous involvement of calpain activity in promoting the cycle at the G(1) checkpoint and somewhere in the G(2)M compartment. Furthermore, the inhibitor significantly impedes the progress of cells through the S phase, indicating calpain activity in S phase checkpoint signalling. Overall, our analysis suggests that calpain regulates the cell cycle at more points than previously thought.

DOI10.1016/j.bcp.2003.12.021
Alternate JournalBiochem. Pharmacol.
PubMed ID15041468