How to determine the efficiency of intermediate transfer in an interacting enzyme system?

TitleHow to determine the efficiency of intermediate transfer in an interacting enzyme system?
Publication TypeJournal Article
Year of Publication1987
AuthorsTompa, P., J. Batke, and J. Ovádi
JournalFEBS Lett
Volume214
Issue2
Pagination244-8
Date Published1987 Apr 20
ISSN0014-5793
KeywordsAspartate Aminotransferases, Enzymes, Glutamate Dehydrogenase, Kinetics, Models, Biological
Abstract

A kinetic method, based upon measuring the transient time of coupled reactions, is proposed for the determination of the intermediate channel efficiency in a system of functionally interacting enzymes. The procedure rests upon a novel description in which the transient time is expressed as a function of channel efficiency and lifetime of the intermediate molecules. By this approach the reduction of transient time can be explained even if no changes in the kinetic parameters of the individual reactions occur. For determining channel efficiency, a linearized form has been evaluated and applied to the analysis of the kinetics of the aspartate aminotransferase-glutamate dehydrogenase coupled reaction, for which the data were taken from the literature [(1982) Eur. J. Biochem. 121, 511-517].

Alternate JournalFEBS Lett.
PubMed ID3569522