SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization.

TitleSAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization.
Publication TypeJournal Article
Year of Publication2012
AuthorsEloy, N. B., N. Gonzalez, J. Van Leene, K. Maleux, H. Vanhaeren, L. De Milde, S. Dhondt, L. Vercruysse, E. Witters, R. Mercier, L. Cromer, G. T. S. Beemster, H. Remaut, M. Van Montagu, G. De Jaeger, P. C. G. Ferreira, and D. Inzé
JournalProc Natl Acad Sci U S A
Volume109
Issue34
Pagination13853-8
Date Published2012 Aug 21
ISSN1091-6490
KeywordsAmino Acid Sequence, Arabidopsis, Cell Cycle, Cyclin A, Gene Expression Regulation, Plant, Models, Biological, Models, Genetic, Molecular Sequence Data, Mutation, Phenotype, Plant Leaves, Plant Proteins, Pollen, Sequence Homology, Amino Acid, Ubiquitin-Protein Ligase Complexes
Abstract

The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana, SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.

DOI10.1073/pnas.1211418109
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID22869741
PubMed Central IDPMC3427114