Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry.

TitleUnraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry.
Publication TypeJournal Article
Year of Publication2008
AuthorsRose, R. J., D. Verger, T. Daviter, H. Remaut, E. Paci, G. Waksman, A. E. Ashcroft, and S. E. Radford
JournalProc Natl Acad Sci U S A
Volume105
Issue35
Pagination12873-8
Date Published2008 Sep 2
Type of Articlesmm
ISSN1091-6490
KeywordsAmino Acid Sequence, Fimbriae, Bacterial, Models, Molecular, Molecular Chaperones, Molecular Sequence Data, Peptides, Protein Subunits, Spectrometry, Mass, Electrospray Ionization
Abstract

P pili are multisubunit fibers essential for the attachment of uropathogenic Escherichia coli to the kidney. These fibers are formed by the noncovalent assembly of six different homologous subunit types in an array that is strictly defined in terms of both the number and order of each subunit type. Assembly occurs through a mechanism termed "donor-strand exchange (DSE)" in which an N-terminal extension (Nte) of one subunit donates a beta-strand to an adjacent subunit, completing its Ig fold. Despite structural determination of the different subunits, the mechanism determining specificity of subunit ordering in pilus assembly remained unclear. Here, we have used noncovalent mass spectrometry to monitor DSE between all 30 possible pairs of P pilus subunits and their Ntes. We demonstrate a striking correlation between the natural order of subunits in pili and their ability to undergo DSE in vitro. The results reveal insights into the molecular mechanism by which subunit ordering during the assembly of this complex is achieved.

DOI10.1073/pnas.0802177105
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID18728178
PubMed Central IDPMC2525559
Grant List / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom