Title | Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Remaut, H., C. Tang, N. S. Henderson, J. S. Pinkner, T. Wang, S. J. Hultgren, D. G. Thanassi, G. Waksman, and H. Li |
Journal | Cell |
Volume | 133 |
Issue | 4 |
Pagination | 640-52 |
Date Published | 2008 May 16 |
Type of Article | smm |
ISSN | 1097-4172 |
Keywords | Amino Acid Sequence, Biosynthetic Pathways, Crystallography, X-Ray, Escherichia coli, Escherichia coli Proteins, Fimbriae Proteins, Fimbriae, Bacterial, Models, Molecular, Molecular Chaperones, Molecular Sequence Data, Multiprotein Complexes, Porins |
Abstract | Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform--the usher--is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 beta strands and is occluded by a folded plug domain, likely gated by a conformationally constrained beta-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria. |
DOI | 10.1016/j.cell.2008.03.033 |
Alternate Journal | Cell |
PubMed ID | 18485872 |
PubMed Central ID | PMC3036173 |
Grant List | 58149 / / Medical Research Council / United Kingdom AI029549 / AI / NIAID NIH HHS / United States AI049950 / AI / NIAID NIH HHS / United States AI48689 / AI / NIAID NIH HHS / United States GM062987 / GM / NIGMS NIH HHS / United States GM074985 / GM / NIGMS NIH HHS / United States R01 AI048689 / AI / NIAID NIH HHS / United States R01 AI048689-08 / AI / NIAID NIH HHS / United States |
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