How a plant lectin recognizes high mannose oligosaccharides.

TitleHow a plant lectin recognizes high mannose oligosaccharides.
Publication TypeJournal Article
Year of Publication2007
AuthorsGarcia-Pino, A., L. Buts, L. Wyns, A. Imberty, and R. Loris
JournalPlant Physiol
Date Published2007 Aug
Type of Articlelectins
KeywordsBinding Sites, Crystallography, X-Ray, Ligands, Mannose, Mannose-Binding Lectins, Molecular Conformation, Oligosaccharides, Pterocarpus, Seeds, Thermodynamics

The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.

Alternate JournalPlant Physiol.
PubMed ID17556509
PubMed Central IDPMC1949895