Title | Expression, purification, crystallization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei. |
Publication Type | Journal Article |
Year of Publication | 1998 |
Authors | Dao-Thi, M-H., T. R. Transue, R. Pellé, N. B. Murphy, F. Poortmans, and J. Steyaert |
Journal | Acta Crystallogr D Biol Crystallogr |
Volume | 54 |
Issue | Pt 5 |
Pagination | 1046-8 |
Date Published | 1998 Sep 1 |
ISSN | 0907-4449 |
Keywords | Animals, Crystallization, Crystallography, X-Ray, Peptidylprolyl Isomerase, Protein Conformation, Protozoan Proteins, Trypanosoma brucei brucei |
Abstract | Cyclophilin A from the bovine parasite Trypanosoma brucei brucei has been cloned, expressed in Escherichia coli, purified and crystallized in the presence of cyclosporin A using ammonium sulfate as a precipitant. The crystals belong to the orthorhombic crystal system with unit-cell dimensions of a = 118.61, b = 210.15 and c = 153.21 A. A data set complete to 2.7 A has been collected using rotating-anode radiation, however the crystals diffract to at least 2.1 A resolution using synchrotron radiation. |
Alternate Journal | Acta Crystallogr. D Biol. Crystallogr. |
PubMed ID | 9757134 |
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