|Title||Therapeutic nanoreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system.|
|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Ranquin, A., W. Versées, W. Meier, J. Steyaert, and P. Van Gelder|
|Date Published||2005 Nov|
|Keywords||Animals, Bacterial Outer Membrane Proteins, Bioreactors, Drug Delivery Systems, Escherichia coli Proteins, Kinetics, N-Glycosyl Hydrolases, Nanostructures, Nanotechnology, Permeability, Polymers, Porins, Prodrugs, Receptors, Virus, Trypanosoma vivax|
Triblock copolymeric nanoreactors are introduced as an alternative for liposomes as encapsulating carrier for prodrug activating enzymes. Inosine-adenosine-guanosine preferring nucleoside hydrolase of Trypanosoma vivax, a potential prodrug activating enzyme, was encapsulated in nanometer-sized vesicles constructed of poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-(2-methyloxazoline) triblock copolymers. The nanoreactor is functionalized by incorporation of bacterial porins, OmpF or Tsx, in the reactor wall. Efficient cleavage of three natural substrates and one prodrug, 2-fluoroadenosine, by the nanoreactors was demonstrated.
|Alternate Journal||Nano Lett.|