Structure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei.

TitleStructure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei.
Publication TypeJournal Article
Year of Publication2010
AuthorsVandemeulebroucke, A., C. Minici, I. Bruno, L. Muzzolini, P. Tornaghi, D. W. Parkin, W. Versées, J. Steyaert, and M. Degano
JournalBiochemistry
Volume49
Issue41
Pagination8999-9010
Date Published2010 Oct 19
ISSN1520-4995
KeywordsAnimals, Crystallography, X-Ray, Kinetics, N-Glycosyl Hydrolases, Nucleosides, Protozoan Proteins, Purinones, Structure-Activity Relationship, Trypanosoma brucei brucei
Abstract

Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are thought to play a primary role in the catabolism of exogenously derived nucleosides. Here, we report the first functional and structural characterization of the inosine-guanosine-specific NH from Trypanosoma brucei brucei. The enzyme shows near diffusion-limited efficiency coupled with a clear specificity for 6-oxopurine nucleosides achieved through a catalytic selection of these substrates. Pre-steady-state kinetic analysis reveals ordered product release, and a rate-limiting structural rearrangement that is associated with the release of the product, ribose. The crystal structure of this trypanosomal NH determined to 2.5 Å resolution reveals distinctive features compared to those of both purine- and pyrimidine-specific isozymes in the framework of the conserved and versatile NH fold. Nanomolar iminoribitol-based inhibitors identified in this study represent important lead compounds for the development of novel therapeutic strategies against trypanosomal diseases.

DOI10.1021/bi100697d
Alternate JournalBiochemistry
PubMed ID20825170