Title | Crystallization and preliminary X-ray analysis of the Man(alpha1-2)Man-specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand. |
Publication Type | Journal Article |
Year of Publication | 2005 |
Authors | Garcia-Pino, A., R. Loris, L. Wyns, and L. Buts |
Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
Volume | 61 |
Issue | Pt 10 |
Pagination | 931-4 |
Date Published | 2005 Oct 1 |
ISSN | 1744-3091 |
Keywords | Amino Acid Sequence, Animals, Carbohydrates, Chromatography, Affinity, Chromatography, Gel, Crystallization, Crystallography, X-Ray, Disaccharides, Fabaceae, Mannose, Mannosides, Models, Statistical, Molecular Sequence Data, Plant Lectins, Protein Conformation, Rabbits, Seeds, Sequence Homology, Amino Acid, Synchrotrons, Temperature, X-Rays |
Abstract | The lectin from Bowringia mildbraedii seeds crystallizes in the presence of the disaccharide Man(alpha1-2)Man. The best crystals grow at 293 K within four weeks after a pre-incubation at 277 K to induce nucleation. A complete data set was collected to a resolution of 1.90 A using synchrotron radiation. The crystals belong to space group I222, with unit-cell parameters a = 66.06, b = 86.35, c = 91.76 A, and contain one lectin monomer in the asymmetric unit. |
DOI | 10.1107/S174430910502854X |
Alternate Journal | Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. |
PubMed ID | 16511199 |
PubMed Central ID | PMC1991310 |
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