Respiratory complex I: 'steam engine' of the cell?

TitleRespiratory complex I: 'steam engine' of the cell?
Publication TypeJournal Article
Year of Publication2011
AuthorsEfremov, R. G., and Sazanov L. A.
JournalCurr Opin Struct Biol
Volume21
Issue4
Pagination532-40
Date Published2011 Aug
ISSN1879-033X
KeywordsAnimals, Cell Membrane, Cells, Electron Transport Complex I, Humans, Hydrophobic and Hydrophilic Interactions, Mitochondria, Protein Structure, Tertiary
Abstract

Complex I is the first enzyme of the respiratory chain and plays a central role in cellular energy production. It has been implicated in many human neurodegenerative diseases, as well as in ageing. One of the biggest membrane protein complexes, it is an L-shaped assembly consisting of hydrophilic and membrane domains. Previously, we have determined structures of the hydrophilic domain in several redox states. Last year was marked by fascinating breakthroughs in the understanding of the complete structure. We described the architecture of the membrane domain and of the entire bacterial complex I. X-ray analysis of the larger mitochondrial enzyme has also been published. The core subunits of the bacterial and mitochondrial enzymes have remarkably similar structures. The proposed mechanism of coupling between electron transfer and proton translocation involves long-range conformational changes, coordinated in part by a long α-helix, akin to the coupling rod of a steam engine.

DOI10.1016/j.sbi.2011.07.002
Alternate JournalCurr. Opin. Struct. Biol.
PubMed ID21831629
Grant List / / Medical Research Council / United Kingdom
Research group: