A census of human soluble protein complexes.

TitleA census of human soluble protein complexes.
Publication TypeJournal Article
Year of Publication2012
AuthorsHavugimana, P. C., T. G Hart, T. Nepusz, H. Yang, A. L. Turinsky, Z. Li, P. I. Wang, D. R. Boutz, V. Fong, S. Phanse, M. Babu, S. A. Craig, P. Hu, C. Wan, J. Vlasblom, V-un-N. Dar, A. Bezginov, G. W. Clark, G. C. Wu, S. J. Wodak, E. R. M. Tillier, A. Paccanaro, E. M. Marcotte, and A. Emili
JournalCell
Volume150
Issue5
Pagination1068-81
Date Published2012 Aug 31
ISSN1097-4172
KeywordsHumans, Multiprotein Complexes, Protein Interaction Maps, Proteins, Proteomics, Tandem Mass Spectrometry
Abstract

Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.

DOI10.1016/j.cell.2012.08.011
Alternate JournalCell
PubMed ID22939629
PubMed Central IDPMC3477804
Grant ListBB/F00964X/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
BB/K004131/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
DP1 GM106408 / GM / NIGMS NIH HHS / United States
MOP 82940 / / Canadian Institutes of Health Research / Canada
R01 GM067779 / GM / NIGMS NIH HHS / United States
R01 GM076536 / GM / NIGMS NIH HHS / United States
R01 GM088624 / GM / NIGMS NIH HHS / United States