|Title||ELP3 controls active zone morphology by acetylating the ELKS family member Bruchpilot.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Miśkiewicz, K., L. E. Jose, A. Bento-Abreu, M. Fislage, I. Taes, J. Kasprowicz, J. Swerts, S. Sigrist, W. Versées, W. Robberecht, and P. Verstreken|
|Date Published||2011 Dec 08|
|Keywords||Acetylation, Acetyltransferases, Animals, Animals, Genetically Modified, Cell Line, Transformed, Drosophila, Drosophila Proteins, Embryo, Mammalian, Green Fluorescent Proteins, Histone Acetyltransferases, Humans, Larva, Microscopy, Electron, Transmission, Mutation, Nerve Tissue Proteins, Neuromuscular Junction, Patch-Clamp Techniques, Presynaptic Terminals, Transfection, Tubulin, Zebrafish|
Elongator protein 3 (ELP3) acetylates histones in the nucleus but also plays a role in the cytoplasm. Here, we report that in Drosophila neurons, ELP3 is necessary and sufficient to acetylate the ELKS family member Bruchpilot, an integral component of the presynaptic density where neurotransmitters are released. We find that in elp3 mutants, presynaptic densities assemble normally, but they show morphological defects such that their cytoplasmic extensions cover a larger area, resulting in increased vesicle tethering as well as a more proficient neurotransmitter release. We propose a model where ELP3-dependent acetylation of Bruchpilot at synapses regulates the structure of individual presynaptic densities and neurotransmitter release efficiency.
ELP3 controls active zone morphology by acetylating the ELKS family member Bruchpilot.